Bacteriophage T5 gene D10 encodes a branch-migration protein

Helicases catalyze the unwinding of double-stranded nucleic acids where structure and phosphate backbone contacts, rather than nucleobase sequence, usually determines substrate specificity. We have expressed and purified a putative helicase encoded by the D10 gene of bacteriophage T5. Here we report that this hitherto uncharacterized protein possesses branch migration and DNA unwinding activity. The initiation of substrate unwinding showed some sequence dependency, while DNA binding and DNA-dependent ATPase activity did not. DNA footprinting and purine-base interference assays demonstrated that D10 engages these substrates with a defined polarity that may be established by protein-nucleobase contacts. Bioinformatic analysis of the nucleotide databases revealed genes predicted to encode proteins related to D10 in archaebacteria, bacteriophages and in viruses known to infect a range of eukaryotic organisms

Aspirin Resistance in Cardiovascular Disease: A Review

AbstractBackground. Aspirin is effective at reducing the cardiovascular event rate in defined patient groups. The introduction of antiplatelet therapies other than aspirin and the concept of aspirin resistance have led to critical reappraisal of current treatment. This review aims to clarify the evidence for aspirin resistance in patients with atherosclerosis.Methods. Medline search was performed to identify publications concerned with antiplatelet effects of aspirin and failure of aspirin therapy. Manual cross referencing was also performed.Results and conclusion. Wide variations in the rate of aspirin resistance (5.5–75%) have been reported. The lack of consensus on an appropriate definition and the number of different tests used to investigate aspirin resistance needs to be addressed. There are few studies where the primary aim was to document aspirin resistance or aspirin non-response. Further work should aim to investigate if aspirin resistance is clinically important and, if it is, what treatments may be beneficial to the at risk patient

Excitation Functions of Stopping Power and Flow in Relativistic Heavy-Ion Collisions

Using a relativistic transport (ART) model, we study the stopping power, the formation of superdense hadronic matter as well as the strength of transverse and radial flow in central Au+Au collisions at beam momentum from 2 to 12 GeV/c per nucleon. We find that complete stopping is achieved in the whole beam momentum range. In particular, the proton rapidity distribution scaled by the beam rapidity is independent of the beam momentum, and this is in agreement with the experimental findings. Also, a large volume of superdense hadronic matter with a local energy density exceeding that expected for the transition of a hadronic matter to the quark-gluon plasma is formed in collisions at beam momenta greater than 8 GeV/c per nucleon. Furthermore, it is found that the transverse flow in these collisions is sensitive to the nuclear equation of state and decreases with increasing beam momentum. On the other hand, the radial flow is insensitive to the equation of state, and its strength increases with beam momentum.Comment: Talk given at NN97, Gatlinburg, Tennessee June 2-6,1997. To appear in the proc. in Nucl. Phys.

Lexical and Prosodic Pitch Modifications in Cantonese Infant-directed Speech

Published online 03 February 2021The functions of acoustic-phonetic modifications in infant-directed speech (IDS) remain a question: do they specifically serve to facilitate language learning via enhanced phonemic contrasts (the hyperarticulation hypothesis) or primarily to improve communication via prosodic exaggeration (the prosodic hypothesis)? The study of lexical tones provides a unique opportunity to shed light on this, as lexical tones are phonemically contrastive, yet their primary cue, pitch, is also a prosodic cue. This study investigated Cantonese IDS and found increased intra-talker variation of lexical tones, which more likely posed a challenge to rather than facilitated phonetic learning. Although tonal space was expanded which could facilitate phonetic learning, its expansion was a function of overall intonational modifications. Similar findings were observed in speech to pets who should not benefit from larger phonemic distinction. We conclude that lexicaltone adjustments in IDS mainly serve to broadly enhance communication rather than specifically increase phonemic contrast for learners.This work was supported by the University Grants Committee (HKSAR) (RGC34000118), the Innovation and Technology Fund (HKSAR) (ITS/067/18), Dr. Stanley Ho Medical Development Foundation, and the Global Parent Child Resource Centre Limited. The second author’s work is supported by the Basque Government through the BERC 2018-2021 program and by the Spanish Ministry of Science and Innovation through the Ramon y Cajal Research Fellowship, PID2019-105528GA-I00

Determinants of antibiotic prescribing for upper respiratory tract infections in an emergency department with good primary care access: a qualitative analysis

Upper respiratory tract infections (URTIs) account for substantial attendances at emergency departments (EDs). There is a need to elucidate determinants of antibiotic prescribing in time-strapped EDs – popular choices for primary care despite highly accessible primary care clinics. Semi-structured in-depth interviews were conducted with purposively sampled physicians (n = 9) in an adult ED in Singapore. All interviews were analysed using thematic analysis and further interpreted using the Social Ecological Model to explain prescribing determinants. Themes included: (1) reliance on clinical knowledge and judgement, (2) patient-related factors, (3) patient–physician relationship factors, (4) perceived practice norms, (5) policies and treatment guidelines and (6) patient education and awareness. The physicians relied strongly on their clinical knowledge and judgement in managing URTI cases and seldom interfered with their peers’ clinical decisions. Despite departmental norms of not prescribing antibiotics for URTIs, physicians would prescribe antibiotics when faced with uncertainty in patients’ diagnoses, treating immunocompromised or older patients with comorbidities, and for patients demanding antibiotics, especially under time constraints. Participants had a preference for antibiotic prescribing guidelines based on local epidemiology, but viewed hospital policies on prescribing as a hindrance to clinical judgement. Participants highlighted the need for more public education and awareness on the appropriate use of antibiotics and management of URTIs. Organisational practice norms strongly influenced antibiotic prescribing decisions by physicians, who can be swayed by time pressures and patient demands. Clinical decision support tools, hospital guidelines and patient education targeting at individual, interpersonal and community levels could reduce unnecessary antibiotic use

The Relationship Between Consumption of Beverages and Tooth Wear Among Adults in The United States

Objectives: To investigate the prevalence and severity of tooth wear (TW) and its relationship with consumption of beverages among adults in the United States. Methods: The National Health and Nutrition Examinations Survey data for 2003-2004 was analyzed. TW was defined as “wear on at least one surface of at least one examined tooth.” Drink/juice consumption was ascertained via a Food Frequency Questionnaire processed with Diet*Calc software to obtain the average daily consumption frequency for all queried drinks and juice categories including milk. Survey-weighted descriptive and multivariable analyses with interaction terms were performed. Results: Our study sample consisted of 3,773 adults (aged 20 and above). Eighty percent of the subjects had evidence of TW, and soft drinks were the most consumed beverage. Significant interactions between the effects of age, gender, and race/ethnicity on TW were found (P \u3c  0.001). After adjustment for demographic factors, consumption of fruit drinks was found to be significantly associated with the severity [odds ratio (OR) = 1.32 and 1.42], but not prevalence of TW. However, this trend was reversed for grape juice consumption (OR = 0.34, 0.41). Conclusions: This study demonstrates that a substantial proportion of adults had evidence of TW, which was affected by demographic factors in a complex way. Fruit drinks consumption in adults was associated with the severity of TW, but not with the prevalence of TW after adjusting for demographics. These findings are important for the development of appropriate treatment guidelines, public policy, and programs aimed at reducing TW in adults

Υ\Upsilon absorption in hadronic matter

The cross sections of $\Upsilon$ absorption by $\pi$ and $\rho$ mesons are evaluated in a meson-exchange model. Including form factors with a cutoff parameter of 1 or 2 GeV, we find that due to the large threshold of these reactions the thermal average of their cross sections is only about 0.2 mb at a temperature of 150 MeV. Our results thus suggest that the absorption of directly produced $\Upsilon$ by hadronic comovers in high energy heavy ion collisions is unimportant.Comment: 11 pages, revtex, 3 figures, added references and discussion on higher BBbar state

Charmonium Absorption in the Meson-exchange Model

We review the meson-exchange model for charmonium absorption by hadrons. This includes the construction of the interaction Lagrangians, the determination of the coupling constants, the introduction of form factors, and the predicted cross sections for $J/\psi$ absorption by both mesons and nucleons. We further discuss the effects due to anomalous parity interactions, uncertainties in form factors, constraints from chiral symmetry, and the change of charmed meson mass in medium on the cross sections for charmonium absorption in hadronic matter.Comment: 10 pages, 2 figures. Talk given at Quark Matter 2002 (QM 2002), Nantes, France, 18-24 July 2002. To appear in the proceedings (Nucl. Phys. A

Excitation functions in central Au+Au collisions from SIS/GSI to AGS/Brookhaven

Using the relativistic transport model (ART), we predict the energy dependence of the stopping power, maximum baryon and energy densities, the population of resonance matter as well as the strength of the transverse and radial flow for central Au+Au reactions at beam momentum from 2 to 12 GeV/c available at Brookhaven's AGS. The maximum baryon and energy densities are further compared to the predictions of relativistic hydrodynamics assuming the formation of shock waves. We also discuss the Fermi-Landau scaling of the pion multiplicity in these reactions.Comment: 20 pages, latex, 10 figures available upon request from the authors, Nucl. Phys. A in pres